MBD2 Antibody

• Background:

  DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD) . Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.

• NCBI Gene ID:

  8932

• Swiss Prot:

  Q9UBB5

• Accession Number:

  Q9UBB5

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This MBD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120-151 amino acids from the N-terminal region of human MBD2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  43 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  MBD2

• NCBI Official Name:

  Methyl-CpG-binding domain protein 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Zhu, Y., et al., Cancer 100 (9) :1853-1858 (2004) .

• Background Reference 02:

  Ghoshal, K., et al., J. Biol. Chem. 279 (8) :6783-6793 (2004) .

• Background Reference 03:

  Fujita, H., et al., Mol. Cell. Biol. 23 (8) :2645-2657 (2003) .

• Background Reference 04:

  Patra, S.K., et al., Biochem. Biophys. Res. Commun. 302 (4) :759-766 (2003) .

• Other Product Names:

  Methyl-CpG-binding domain protein 2, Demethylase, DMTase, Methyl-CpG-binding protein MBD2, MBD2

• Tested Applications:

  WB

• Protein ID:

  50401198

• Physical Properties:

  Liquid