Mouse Cblb Antibody

• Background :

  E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. May also be involved in EGFR ubiquitination and internalization.

• NCBI Gene ID :

  208650

• Swiss Prot :

  Q3TTA7

• Accession Number :

  Q3TTA7

• Host :

  Rabbit

• Reactivity :

  Mouse

• Immunogen :

  This Mouse Cblb antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 862-889 amino acids from the C-terminal region of mouse Cblb.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Cancer, Cell Cycle, Immunology

• Purification :

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration :

  Batch dependent

• Buffer :

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight :

  109 kDa

• Fragment :

  Rabbit Ig

• Applications Notes :

  For WB starting dilution is: 1:1000

• Symbol :

  Cblb

• NCBI Official Name :

  E3 ubiquitin-protein ligase CBL-B

• NCBI Organism :

  Mus musculus

• Background Reference 01 :

  Wilson, B.G., et al. Cancer Cell 18 (4) :316-328 (2010)

• Background Reference 02 :

  Stromnes, I.M., et al. J. Clin. Invest. 120 (10) :3722-3734 (2010)

• Background Reference 03 :

  Naramura, M., et al. Proc. Natl. Acad. Sci. U.S.A. 107 (37) :16274-16279 (2010)

• Background Reference 04 :

  Teh, C.E., et al. Proc. Natl. Acad. Sci. U.S.A. 107 (33) :14709-14714 (2010)

• Other Product Names :

  E3 ubiquitin-protein ligase CBL-B, 632-, Casitas B-lineage lymphoma proto-oncogene b, SH3-binding protein CBL-B, Signal transduction protein CBL-B, Cblb

• Tested Applications :

  WB

• Physical Properties :

  Liquid