CBLB / RNF56 Recombinant Protein

• Background :

  Casitas B-lineage lymphoma proto-oncogene b (CBLB) is also known as RNF56. E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. Expressed in placenta, heart, lung, kidney, spleen, ovary and testis, as well as fetal brain and liver and hematopoietic cell lines, but not in adult brain, liver, pancreas, salivary gland, or skeletal muscle.

• NCBI Gene ID :

  868

• Swiss Prot :

  Q13191

• Accession Number :

  NP_733762.2

• Tag :

  His Tag

• Type :

  Recombinant Proteins

• Source :

  E. coli

• Purity :

  >95% as determined by SDS-PAGE.

• Buffer :

  40 mM HEPES, 120 mM NaCl, pH7.5

• Shipping Conditions :

  Room Temp

• Storage Conditions :

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight :

  47.0 kDa

• Applications Notes :

  This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 47.0 kDa. The protein migrates as 46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name :

  Cblproto-oncogeneB

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Xiao Y., et al., 2015, J Immunol., 195 (9) : 4218-4227.

• Background Reference 02 :

  Desai SS., et al., 2015, J Biol Chem., 290 (42) : 25595-25608.

• Background Reference 03 :

  Van de Veerdonk FL., et al., 2016, Nat Med., 22 (8) : 834-835.

• Other Product Names :

  CBL-B, CBLB, RNF56

• Tested Applications :

  WB

• Species :

  Human

• Physical Properties :

  Lyophilized

• CAS Number :

  9000-83-3