NR1H4 Antibody

• Background:

  NR1H4 is a Ligand-activated transcription factor. Receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. Represses the transcription of the cholesterol 7-alpha-hydroxylase gene (CYP7A1) through the induction of NR0B2 or FGF19 expression, via two distinct mechanisms. Activates the intestinal bile acid-binding protein (IBABP) . Activates the transcription of bile salt export pump ABCB11 by directly recruiting histone methyltransferase CARM1 to this locus.

• NCBI Gene ID:

  9971

• Swiss Prot:

  Q96RI1

• Accession Number:

  Q96RI1

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This NR1H4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-46 amino acids from the N-terminal region of human NR1H4.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Obesity, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  56 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  NR1H4

• NCBI Official Name:

  Bile acid receptor

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Meyer Zu Schwabedissen, H.E., et al. Hepatology 52 (5) :1797-1807 (2010)

• Background Reference 02:

  Popescu, I.R., et al. FEBS Lett. 584 (13) :2845-2851 (2010)

• Background Reference 03:

  Gadaleta, R.M., et al. Biochim. Biophys. Acta 1801 (7) :683-692 (2010)

• Background Reference 04:

  Roberts, K.E., et al. Gastroenterology 139 (1) :130-139 (2010)

• Other Product Names:

  Bile acid receptor, Farnesoid X-activated receptor, Farnesol receptor HRR-1, Nuclear receptor subfamily 1 group H member 4, Retinoid X receptor-interacting protein 14, RXR-interacting protein 14, NR1H4, BAR, FXR, HRR1, RIP14

• Tested Applications:

  WB

• Protein ID:

  46577705

• Physical Properties:

  Liquid