Mouse Src Antibody

• Background:

  Non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. Once it is recruited to the activated integrins, by PTK2B, it phosphorylates CBL which in turn induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. Promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. Phosphorylates RUNX3 and COX2 on tyrosine residues, TNK2 on 'Tyr-284' and CBL on 'Tyr-731'. Enhances DDX58/RIG-I-elicited antiviral signaling.

• NCBI Gene ID:

  20779

• Swiss Prot:

  P05480

• Accession Number:

  P05480

• Host:

  Rabbit

• Reactivity:

  Mouse

• Immunogen:

  This Mouse Src antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-125 amino acids from the N-terminal region of mouse Src.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  61 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  Src

• NCBI Official Name:

  Neuronal proto-oncogene tyrosine-protein kinase Src

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Tanji, M., et al. Mol. Cell. Biol. 30 (19) :4604-4615 (2010)

• Background Reference 02:

  Wang, L., et al. J. Neurosci. 30 (39) :13201-13210 (2010)

• Background Reference 03:

  Tu, C., et al. Proc. Natl. Acad. Sci. U.S.A. 107 (37) :16107-16112 (2010)

• Background Reference 04:

  Zheng, Y., et al. Mol. Cell. Biol. 30 (17) :4280-4292 (2010)

• Other Product Names:

  Neuronal proto-oncogene tyrosine-protein kinase Src, Proto-oncogene c-Src, pp60c-src, p60-Src, Src

• Tested Applications:

  WB

• Physical Properties:

  Liquid