Src (Ab 529) Antibody

CAT: 0223-79-345Size: 0.1 mgDry Ice: NoHazardous: No
CAT#:0223-79-345Size:0.1 mg
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Background
Proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme.
NCBI Gene ID
6714
Swiss Prot
P12931
Accession Number
NP_005408.1
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Src (Ab-529) antibody was raised against a peptide sequence around aa. 527~531 (P-Q-Y-Q-P) derived from Human Src.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Apoptosis, Cancer
Purification
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Calculated Molecular Weight
60 kDa
Specificity
This antibody detects endogenous level of total Src protein.
Applications Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Symbol
SRC
NCBI Official Name
V-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
NCBI Organism
Homo sapiens
Background Reference 01
Pyper J. M., (1985) Mol. Cell. Biol. 5:831-838Pyper J. M. (1990) Mol. Cell. Biol. 10:2035-2040Xu W., (1997) . Nature 385:595-602Benes C. H., (2005) Cell 121:271-280
Other Product Names
ASV, SRC1, c-SRC, p60-Src, ZFYVE2, C-SRC
Tested Applications
WB, IHC
Protein ID
4885609
Physical Properties
Liquid

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