DIAPH3 Antibody

• Background:

  DIAPH3 binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (By similarity) .

• NCBI Gene ID:

  81624

• Swiss Prot:

  Q9NSV4

• Accession Number:

  Q9NSV4

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This DIAPH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1061-1090 amino acids from the C-terminal region of human DIAPH3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  137 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  DIAPH3

• NCBI Official Name:

  Protein diaphanous homolog 3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  DeWard, A.D., et al. J. Biol. Chem. 284 (30) :20061-20069 (2009)

• Background Reference 02:

  Block, J., et al.Microsc 231 (3) :506-517 (2008)

• Background Reference 03:

  Beli, P., et al. Nat. Cell Biol. 10 (7) :849-857 (2008)

• Background Reference 04:

  Yang, C., et al. PLoS Biol. (11), E317 (2007) :

• Other Product Names:

  Protein diaphanous homolog 3, Diaphanous-related formin-3, DRF3, MDia2, DIAPH3, DIAP3

• Tested Applications:

  WB

• Protein ID:

  158520000

• Physical Properties:

  Liquid