MCM8 Antibody

• Background:

  MCM8 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein contains the central domain that is conserved among the MCM proteins. This protein has been shown to co-immunoprecipitate with MCM4, 6 and 7, which suggests that it may interact with other MCM proteins and play a role in DNA replication. Alternatively spliced transcript variants encoding distinct isoforms have been described.

• NCBI Gene ID:

  84515

• Swiss Prot:

  Q9UJA3

• Accession Number:

  NP_115874

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MCM8.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store MCM8 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  92 kDa, 92 kDa

• Applications Notes:

  MCM8 antibody can be used for detection of MCM8 by ELISA at 1:62500. MCM8 antibody can be used for detection of MCM8 by western blot at 0.125 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  MCM8

• NCBI Official Name:

  Minichromosome maintenance complex component 8

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Volkening, M., et al., (2005) Mol. Cell. Biol. 25 (4), 1560-1568.

• Other Product Names:

  MCM8, C20orf154, dJ967N21.5

• Tested Applications:

  ELISA, WB

• Protein ID:

  19923727

• Physical Properties:

  Liquid