MCM4 Antibody

CAT: 0223-28-536Size: 100 µLDry Ice: NoHazardous: No
CAT#:0223-28-536Size:100 µL
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Background
The protein encoded by MCM4 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. The MCM4 gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks.
NCBI Gene ID
4173
Swiss Prot
P33991
Accession Number
NP_005905
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MCM4.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cell Cycle, Cancer
Purification
Antibody is purified by protein A chromatography method.
Positive Control
Cat. No. 1205 - Jurkat Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store MCM4 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
95 kDa, 97 kDa
Applications Notes
MCM4 antibody can be used for detection of MCM4 by ELISA at 1:12500. MCM4 antibody can be used for detection of MCM4 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
MCM4
NCBI Official Name
Minichromosome maintenance complex component 4
NCBI Organism
Homo sapiens
Background Reference 01
Ishimi, Y., et al., (2003) J. Biol. Chem. 278 (27), 24644-24650.
Other Product Names
MCM4, NKCD, CDC21, CDC54, NKGCD, hCdc21, P1-CDC21
Tested Applications
ELISA, WB
Protein ID
33469919
Physical Properties
Liquid

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