HAT1 Antibody

• Background:

  The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene.

• NCBI Gene ID:

  8520

• Swiss Prot:

  O14929

• Accession Number:

  O14929

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This HAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-50 amino acids from the N-terminal region of human HAT1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  50 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  HAT1

• NCBI Official Name:

  Histone acetyltransferase type B catalytic subunit

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Saade, E., et al. Proteomics 9 (21) :4934-4943 (2009)

• Background Reference 02:

  Miyamoto, N., et al. J. Biol. Chem. 283 (26) :18218-18226 (2008)

• Background Reference 03:

  Sugiyama, N., et al. Mol. Cell Proteomics 6 (6) :1103-1109 (2007)

• Background Reference 04:

  Lamesch, P., et al. Genomics 89 (3) :307-315 (2007)

• Other Product Names:

  Histone acetyltransferase type B catalytic subunit, Histone acetyltransferase 1, HAT1, KAT1

• Tested Applications:

  WB

• Physical Properties:

  Liquid