MCM4 Antibody

• Background:

  The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.

• NCBI Gene ID:

  4173

• Swiss Prot:

  P33991

• Accession Number:

  P33991

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This MCM4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 637-665 amino acids from the C-terminal region of human MCM4.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Xenopus, Mouse

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  97 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  MCM4

• NCBI Official Name:

  DNA replication licensing factor MCM4

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :

• Background Reference 02:

  Qian, Z., et al. PLoS Pathog. (3), E1000814 (2010) :

• Background Reference 03:

  Ladstein, R.G., et al. BMC Cancer 10, 140 (2010) :

• Background Reference 04:

  Hosgood, H.D. III, et al. Occup Environ Med 66 (12) :848-853 (2009)

• Other Product Names:

  DNA replication licensing factor MCM4, CDC21 homolog, P1-CDC21, MCM4, CDC21

• Tested Applications:

  WB, IHC-P

• Protein ID:

  68571766

• Physical Properties:

  Liquid