Mouse Bckdk Antibody

• Background:

  Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex (By similarity) .

• NCBI Gene ID:

  12041

• Swiss Prot:

  O55028

• Accession Number:

  O55028

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This Mouse Bckdk antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-188 amino acids from the Central region of mouse Bckdk.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  47 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  Bckdk

• NCBI Official Name:

  [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Pagliarini, D.J., et al. Cell 134 (1) :112-123 (2008)

• Background Reference 02:

  Lee, J., et al. Mol. Cell Proteomics 6 (4) :669-676 (2007)

• Background Reference 03:

  Hutson, S.M. Biochem. J. 400 (1), E1-E3 (2006) :

• Background Reference 04:

  Joshi, M.A., et al. Biochem. J. 400 (1) :153-162 (2006)

• Other Product Names:

  [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial, Branched-chain alpha-ketoacid dehydrogenase kinase, BCKD-kinase, BCKDHKIN, Bckdk

• Tested Applications:

  WB

• Physical Properties:

  Liquid