PHAX Antibody

• Background:

  A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC) -bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP-and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner (By similarity) . Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA) . Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG) -capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA.

• NCBI Gene ID:

  51808

• Swiss Prot:

  Q9H814

• Accession Number:

  Q9H814

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This PHAX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 297-326 amino acids from the C-terminal region of human PHAX.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  44 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  PHAX

• NCBI Official Name:

  Phosphorylated adapter RNA export protein

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Mourao, A., et al. RNA 16 (6) :1205-1216 (2010)

• Background Reference 02:

  Matsuoka, S., et al. Science 316 (5828) :1160-1166 (2007)

• Background Reference 03:

  Lemm, I., et al. Mol. Biol. Cell 17 (7) :3221-3231 (2006)

• Background Reference 04:

  Watkins, N.J., et al. Mol. Cell 16 (5) :789-798 (2004)

• Other Product Names:

  Phosphorylated adapter RNA export protein, RNA U small nuclear RNA export adapter protein, PHAX, RNUXA

• Tested Applications:

  WB, IHC-P

• Protein ID:

  74752718

• Physical Properties:

  Liquid