PHAP Antibody

• Background:

  PHAP Antibody: Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.

• NCBI Gene ID:

  8125

• Swiss Prot:

  P39687

• Accession Number:

  P39687

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Apoptosis

• Purification:

  PHAP Antibody is DEAE purified.

• Positive Control:

  Cat. No. 1207 - Raji Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine: (85%)

• Buffer:

  PHAP Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  PHAP antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  35 kDa

• Fragment:

  IgG

• Symbol:

  ANP32A

• Positive Control 2:

  Cat. No. 1308 - Human Small Intestine Tissue Lysate

• Positive Control 3:

  Cat. No. 11-801 - Human Small Intestine Tissue Slide

• NCBI Official Name:

  Acidic (leucine-rich) nuclear phosphoprotein 32 family, member A

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Jiang X, Kim HE, Shu H, Zhao Y, Zhang H, Kofron J, Donnelly J, Burns D, Ng SC , Rosenberg S, Wang X. Distinctive roles of PHAP proteins and prothymosin-α in a death regulatory pathway. Science. 2003;299 (5604) :223-6.

• Background Reference 02:

  Nicholson DW, Thornberry NA. Apoptosis. Life and death decisions. Science. 2003 10;299 (5604) :214-5.

• Other Product Names:

  PHAP Antibody: LANP, MAPM, PP32, HPPCn, PHAP1, PHAPI, I1PP2A, C15orf1, LANP, Acidic leucine-rich nuclear phosphoprotein 32 family member A, Acidic nuclear phosphoprotein pp32

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  730318

• Physical Properties:

  Liquid