PLOD3 Antibody

• Background:

  The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity.

• NCBI Gene ID:

  8985

• Swiss Prot:

  O60568

• Accession Number:

  O60568

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This PLOD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 78-105 amino acids from the N-terminal region of human PLOD3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  85 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  PLOD3

• NCBI Official Name:

  Procollagen-lysine,2-oxoglutarate 5-dioxygenase 3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Wang, C., et al. J. Cell. Mol. Med. 13 (3) :508-521 (2009)

• Background Reference 02:

  Salo, A.M., et al. Am. J. Hum. Genet. 83 (4) :495-503 (2008)

• Background Reference 03:

  Salo, A.M., et al. J. Cell. Physiol. 207 (3) :644-653 (2006)

• Background Reference 04:

  Wang, C., et al. Matrix Biol. 21 (7) :559-566 (2002)

• Other Product Names:

  Procollagen-lysine,2-oxoglutarate 5-dioxygenase 3, Lysyl hydroxylase 3, LH3, PLOD3

• Tested Applications:

  WB

• Physical Properties:

  Liquid