Mdm2 Antibody

• Background:

  MDM2 is a target of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.

• NCBI Gene ID:

  4193

• Swiss Prot:

  Q00987

• Accession Number:

  Q00987

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This Mdm2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 393-424 amino acids from the C-terminal region of human Mdm2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  55 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  MDM2

• NCBI Official Name:

  E3 ubiquitin-protein ligase Mdm2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Burch, L.R., et al., J. Mol. Biol. 337 (1) :115-128 (2004) . Schon, O., et al., J. Mol. Biol. 336 (1) :197-202 (2004) . Mantesso, A., et al., J. Oral Pathol. Med. 33 (2) :96-101 (2004) . Shmueli, A., et al., Mol. Cell 13 (1) :4-5 (2004) . Xia, L., et al., Cancer Res. 64 (1) :221-228 (2004) .

• Other Product Names:

  E3 ubiquitin-protein ligase Mdm2, 632-, Double minute 2 protein, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2, MDM2

• Tested Applications:

  WB, IHC-P

• Protein ID:

  266516

• Physical Properties:

  Liquid