HLA-DQA1 Antibody

• Background:

  HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages) . The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.

• NCBI Gene ID:

  1005094573117

• Swiss Prot:

  P01909

• Accession Number:

  P01909

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This HLA-DQA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-84 amino acids from the Central region of human HLA-DQA1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Immunology

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  28 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  HLA-DQA1

• NCBI Official Name:

  HLA class II histocompatibility antigen, DQ alpha 1 chain

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Donaldson, P.T., et al. J. Hepatol. 53 (6) :1049-1053 (2010)

• Background Reference 02:

  Noble, J.A., et al. Diabetes 59 (11) :2972-2979 (2010)

• Background Reference 03:

  Ferreira, R.C., et al. Nat. Genet. 42 (9) :777-780 (2010)

• Background Reference 04:

  Ovsyannikova, I.G., et al. PLoS ONE (7), E11806 (2010) :

• Other Product Names:

  HLA class II histocompatibility antigen, DQ alpha 1 chain, DC-1 alpha chain, DC-alpha, HLA-DCA, MHC class II DQA1, HLA-DQA1

• Tested Applications:

  WB, Flow

• Protein ID:

  122188

• Physical Properties:

  Liquid