MED7 Antibody

• Background:

  The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].

• NCBI Gene ID:

  9443

• Swiss Prot:

  O43513

• Accession Number:

  O43513

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This MED7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 83-111 amino acids from the Central region of human MED7.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  27 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  MED7

• NCBI Official Name:

  Mediator of RNA polymerase II transcription subunit 7

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :

• Background Reference 02:

  Sato, S., et al. Mol. Cell 14 (5) :685-691 (2004)

• Background Reference 03:

  Tomomori-Sato, C., et al. J. Biol. Chem. 279 (7) :5846-5851 (2004)

• Background Reference 04:

  Sato, S., et al. J. Biol. Chem. 278 (17) :15123-15127 (2003)

• Other Product Names:

  Mediator of RNA polymerase II transcription subunit 7, hMED7, Activator-recruited cofactor 34 kDa component, ARC34, Cofactor required for Sp1 transcriptional activation subunit 9, CRSP complex subunit 9, Mediator complex subunit 7, RNA polymerase transcriptional regulation mediator subunit 7 homolog, Transcriptional coactivator CRSP33, MED7, ARC34, CRSP9

• Tested Applications:

  WB, Flow

• Physical Properties:

  Liquid