MED6 Antibody

• Background:

  MED6 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. And mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

• NCBI Gene ID:

  10001

• Swiss Prot:

  O75586

• Accession Number:

  NP_005457

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MED6.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Signal Transduction

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  721_B Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store MED6 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  28 kDa

• Applications Notes:

  MED6 antibody can be used for detection of MED6 by ELISA at 1:312500. MED6 antibody can be used for detection of MED6 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  MED6

• NCBI Official Name:

  Mediator complex subunit 6

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Pavri, R., (2005) Mol. Cell 18 (1), 83-96.

• Other Product Names:

  MED6, NY-REN-28, ARC33

• Tested Applications:

  ELISA, WB

• Protein ID:

  42544155

• Physical Properties:

  Liquid