SET07 Antibody

• Background:

  SET07 is a histone methyltransferase that methylates Lys-20 of histone H4. H4 Lys-20 methylation represents a specific tag for epigenetic transcriptional repression. The nuclear SET07 protein, which associates with silent chromatin on euchromatic arms but shows no association with constitutive heterochromatin, prefers nucleosomes as substrate compared to free histones. It appears that SET07 may play a role in maintaining silent chromatin by preventing neighboring acetylation of H4 tail. Although the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity.

• NCBI Gene ID:

  387893

• Swiss Prot:

  Q9NQR1

• Accession Number:

  Q9NQR1

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This SET07 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 362-393 amino acids from the C-terminal region of human SET07.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  43 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  SETD8

• NCBI Official Name:

  N-lysine methyltransferase SETD8

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Nishioka, K., et al., Mol. Cell 9 (6) :1201-1213 (2002) .

• Background Reference 02:

  Fang, J., et al., Curr. Biol. 12 (13) :1086-1099 (2002) .

• Other Product Names:

  N-lysine methyltransferase SETD8, 211-, H4-K20-HMTase SETD8, Histone-lysine N-methyltransferase SETD8, Lysine N-methyltransferase 5A, PR/SET domain-containing protein 07, PR-Set7, PR/SET07, SET domain-containing protein 8, SETD8, KMT5A, PRSET7, SET07, SET8

• Tested Applications:

  WB

• Protein ID:

  25091219

• Physical Properties:

  Liquid