SETD7 Antibody

• Background:

  SETD7 is a histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. It plays a central role in the transcriptional activation of genes such as collagenase or insulin. It is recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. It has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins.

• NCBI Gene ID:

  80854

• Swiss Prot:

  Q8WTS6

• Accession Number:

  NP_085151

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SETD7.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Fetal Pancreas Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SETD7 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  41 kDa

• Applications Notes:

  SETD7 antibody can be used for detection of SETD7 by ELISA at 1:312500. SETD7 antibody can be used for detection of SETD7 by western blot at 5.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  SETD7

• NCBI Official Name:

  SET domain containing (lysine methyltransferase) 7

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Subramanian, K., (2008) Mol. Cell 30 (3), 336-347.

• Other Product Names:

  SETD7, FLJ21193, KIAA1717, KMT7, SET7, SET7/9, SET9

• Tested Applications:

  ELISA, WB

• Protein ID:

  18139549

• Physical Properties:

  Liquid