SET7 Antibody

• Background:

  Histone methyltransferases (HMTases) selectively methylate evolutionarily conserved arginine or lysine residues, primarily in the N-terminal tails of histones H3 and H4. Signal transduction pathways affecting the N-terminal tails of histones lead to a number of post-translational modifications including acetylation, phosphorylation, poly (ADP-ribosylation), ubiquitination and methylation. These modifications play critical roles in regulating chromatin structure and gene expression. Set7/9 is a histone specific HMTase that methylates histone H3 lysine 4. Set7/9 transfers methyl groups to lysine 4 of histone H3 in complex with S-adenosyl-L-methionine. In yeast, H4-K20 methylation does not have any apparent role in the regulation of gene expression or heterochromatin function; rather it appears to play a role in DNA damage response. Loss of Set9 activity or mutation of H4-K20 markedly impairs yeast cell survival after genotoxic challenge and compromises the ability of cells to maintain checkpoint mediated cell cycle arrest. Genetic experiments link Set9 to Crb2, a homolog of the mammalian checkpoint protein 53BP1, and the enzyme is required for Crb2 localization to sites of DNA damage.

• NCBI Gene ID:

  80854

• Swiss Prot:

  Q8WTS6

• Accession Number:

  Q8WTS6

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This SET7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 289-317 amino acids from the C-terminal region of human SET7.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  41 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  SETD7

• NCBI Official Name:

  Histone-lysine N-methyltransferase SETD7

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Chuikov, S., et al., Nature 432 (7015) :353-360 (2004) .

• Background Reference 02:

  Wysocka, J., et al., Genes Dev. 17 (7) :896-911 (2003) .

• Background Reference 03:

  Xiao, B., et al., Nature 421 (6923) :652-656 (2003) .

• Background Reference 04:

  Kwon, T., et al., EMBO J. 22 (2) :292-303 (2003) .

• Other Product Names:

  Histone-lysine N-methyltransferase SETD7, Histone H3-K4 methyltransferase SETD7, H3-K4-HMTase SETD7, Lysine N-methyltransferase 7, SET domain-containing protein 7, SET7/9, SETD7, KIAA1717, KMT7, SET7, SET9

• Tested Applications:

  WB

• Protein ID:

  25091217

• Physical Properties:

  Liquid