PARP-10 Antibody [5H11]

• Background:

  PARP-10. PARP-10 mono (ADP-ribosyl) ates (MARylates) NEMO (NF-kappaB essential modulator), which results in reduced NEMO polyubiquitylation and thus decreased NF-kappaB signaling. Overexpression of PARP-10 was shown to lead to apoptosis. PARP-10 knockdown leads to increased cell survival. Additional substrates of PARP-10, such as GSK-3beta were identified and PARP-10-mediated mono (ADP-ribosyl) ation (MARylation) was shown to be involved in regulating multiple processes, including Wnt signaling.

• NCBI Gene ID:

  84875

• Swiss Prot:

  Q53GL7

• Accession Number:

  116248563

• Host:

  Rat

• Reactivity:

  Human

• Immunogen:

  Recombinant human PARP-10 [ARTD10] (aa 1-907) fused to a GST-tag.

• Clonality:

  Monoclonal

• Clone:

  5H11

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Apoptosis, Cancer, Innate Immunity, Obesity

• Concentration:

  Batch dependent

• Buffer:

  Liquid. Cell culture supernatant containing RPMI, 10% FCS and 0.05% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Stable for at least 1 year after receipt when stored at +4˚ C.

• Fragment:

  IgG1

• Specificity:

  Recognizes human PARP-10 [ARTD10] with an epitope between aa 256-407, containing the glycine rich (G-rich) region.

• Applications Notes:

  Immunocytochemistry: (1:50-1:200) . Immunoprecipitation: (1:10) . Western Blot: (1:50) . Optimal conditions must be determined individually for each application.

• Symbol:

  PARP10

• NCBI Official Name:

  Poly (ADP-ribose) polymerase family member 10

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  ADP-ribosyltransferase Diphtheria Toxin-like 10; Poly (ADP-ribose) Polymerase-10

• Tested Applications:

  ICC, IP, WB

• Protein ID:

  Q53GL7

• Physical Properties:

  Liquid