PRKAA2 Antibody

• Background :

  The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK) . AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

• NCBI Gene ID :

  5563

• Swiss Prot :

  P54646

• Accession Number :

  P54646

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  This PRKAA2 (Thr172) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-173 amino acids from the Central region of human PRKAA2 (Thr172) .

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Cancer, Obesity, Neuroscience, Signal Transduction, Autophagy

• Purification :

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration :

  Batch dependent

• Homology :

  Predicted species reactivity based on immunogen sequence: Mouse, Pig, Rat

• Buffer :

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight :

  62 kDa

• Fragment :

  Rabbit Ig

• Symbol :

  PRKAA2

• NCBI Official Name :

  5'-AMP-activated protein kinase catalytic subunit alpha-2

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Jablonski, K.A., et al. Diabetes 59 (10) :2672-2681 (2010)

• Background Reference 02 :

  Bailey, S.D., et al. Diabetes Care 33 (10) :2250-2253 (2010)

• Background Reference 03 :

  Jassim, G., et al. Pharmacopsychiatry (2010) In press :

• Background Reference 04 :

  Bungard, D., et al. Science 329 (5996) :1201-1205 (2010)

• Other Product Names :

  5'-AMP-activated protein kinase catalytic subunit alpha-2, AMPK subunit alpha-2, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, PRKAA2, AMPK, AMPK2

• Tested Applications :

  WB, IHC-P, Flow

• Protein ID :

  20178276

• Physical Properties :

  Liquid