HLA-B Antibody

• Background:

  HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin) . The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.

• NCBI Gene ID:

  3106

• Swiss Prot:

  P01889

• Accession Number:

  P01889

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This HLA-B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-208 amino acids from the Central region of human HLA-B.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Immunology

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  40 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  HLA-B

• NCBI Official Name:

  HLA class I histocompatibility antigen, B-7 alpha chain

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Parsons, M.S., et al. J. Infect. Dis. 202 SUPPL 3, S356-S360 (2010) :

• Background Reference 02:

  Brockman, M.A., et al. J. Virol. 84 (22) :11937-11949 (2010)

• Background Reference 03:

  Noble, J.A., et al. Diabetes 59 (11) :2972-2979 (2010)

• Background Reference 04:

  Lipponen, K., et al. Diabetes (2010) In press :

• Other Product Names:

  HLA class I histocompatibility antigen, B-7 alpha chain, MHC class I antigen B*7, HLA-B, HLAB

• Tested Applications:

  WB

• Protein ID:

  122162

• Physical Properties:

  Liquid