HLA-E Antibody

• Background :

  HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin) . The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.

• NCBI Gene ID :

  3133

• Swiss Prot :

  P13747

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HLA-E (NP_005507.3) .

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Immunology

• Purification :

  Affinity purification

• Positive Control :

  SKOV3

• Concentration :

  Batch dependent

• Buffer :

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store at -20˚ C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight :

  Observed: 45kDa

• Fragment :

  IgG

• Applications Notes :

  WB: 1:500 - 1:2000

• Symbol :

  HLA-E

• Positive Control 2 :

  Jurkat

• Positive Control 3 :

  MCF7

• Positive Control 4 :

  HL-60

• Positive Control 5 :

  SW480

• NCBI Official Name :

  Major histocompatibility complex, class I, E

• NCBI Organism :

  Homo sapiens

• Other Product Names :

  HLA-E, EA2.1, HLAE, MHC HLA-E alpha-2.1, MHC HLA-E alpha-1, EA1.2, HLA-6.2, MHC class I antigen E, QA1

• Tested Applications :

  WB

• Physical Properties :

  Liquid