GRP94 Antibody [SRPR90b]

• Background:

  Heat shock protein 90kDa beta member 1, known also as endoplasmin, gp96, grp94 and ERp99, is an HSP90 paralogue that is found in the endoplasmic reticulum. It plays critical roles in folding proteins in the secretory pathway such as Toll-like receptors and integrins. It has been implicated as an essential immune chaperone to regulate both innate and adaptive immunity. [Wiki]

• NCBI Gene ID:

  7184

• Swiss Prot:

  P14625

• Host:

  Rat

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human HSP90B1 protein was used as the immunogen for the GRP94 antibody.

• Clonality:

  Monoclonal

• Clone:

  SRPR90b

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Obesity, Signal Transduction

• Purification:

  Protein G affinity chromatography

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS with 0.1 mg/ml BSA and 0.05% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

• Fragment:

  IgG2a, kappa

• Applications Notes:

  1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• Symbol:

  HSP90B1

• NCBI Official Name:

  Heat shock protein 90kDa beta (Grp94), member 1

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  Endoplasmin, 94 kDa glucose-regulated protein, GRP-94, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1, gp96 homolog, HSP90B1, GRP94, TRA1

• Tested Applications:

  WB, IF, IHC-P

• Physical Properties:

  Liquid