GRP94 Antibody [HSP90B1/1192]

• Background:

  Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96) . Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90) . This mAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grps are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.

• NCBI Gene ID:

  7184

• Swiss Prot:

  P14625

• Host:

  Rat

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human protein was used as the immunogen for the GRP94 antibody.

• Clonality:

  Monoclonal

• Clone:

  HSP90B1/1192

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Obesity, Signal Transduction

• Purification:

  Protein G affinity chromatography

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS with 0.1 mg/ml BSA and 0.05% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

• Fragment:

  IgG2a, kappa

• Applications Notes:

  1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• Symbol:

  HSP90B1

• NCBI Official Name:

  Heat shock protein 90kDa beta (Grp94), member 1

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  Endoplasmin, 94 kDa glucose-regulated protein, GRP-94, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1, gp96 homolog, HSP90B1, GRP94, TRA1

• Tested Applications:

  Flow, IF, WB, IHC-P

• Physical Properties:

  Liquid