TIMP3 Antibody

• Background:

  TIMP3 (tissue inhibitor of metalloproteinases 3), along with family members TIMP1, TIMP2, and TIMP4, are inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix (ECM) . An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors. TIMP’s inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. TIMP3 may be involved in regulating trophoblastic invasion of the uterus as well as in regulating remodeling of the extracellular matrix during the folding of epithelia, and in the formation, branching and expansion of epithelial tubes.

• NCBI Gene ID:

  7078

• Swiss Prot:

  P35625

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Horse, Bovine, Dog

• Immunogen:

  Amino acids 175-211 of human TIMP3 Ab was used as the immunogen for this antibody (100% homologous in cow, dog and horse, and 94% homologous in mouse and rat) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle, Neuroscience, Signal Transduction

• Purification:

  Protein A affinity chromatography

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS with 0.1 mg/ml BSA and 0.05% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

• Fragment:

  IgG

• Applications Notes:

  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TIMP3 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• Symbol:

  TIMP3

• NCBI Official Name:

  Metalloproteinase inhibitor 3

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  Metalloproteinase inhibitor 3, Protein MIG-5, Tissue inhibitor of metalloproteinases 3, TIMP-3, TIMP3

• Tested Applications:

  WB, Flow, IHC, IP, IF

• Physical Properties:

  Liquid