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DNMT1 Antibody

CAT: 0223-55-132Size: 400 µLDry Ice: NoHazardous: No
CAT#:0223-55-132Size:400 µL
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2Fbinding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells.
NCBI Gene ID
1786
Swiss Prot
P26358
Accession Number
P26358
Host
Rabbit
Reactivity
Human
Immunogen
This DNMT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1083-1112 amino acids from human DNMT1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Batch dependent
Homology
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
183 kDa
Fragment
Rabbit Ig
Applications Notes
For WB starting dilution is: 1:1000
Symbol
DNMT1
NCBI Official Name
DNA (cytosine-5) -methyltransferase 1
NCBI Organism
Homo sapiens
Background Reference 01
Peterson, E.J., et al., Cancer Res. 63 (20) :6579-6582 (2003) .
Background Reference 02
Leu, Y.W., et al., Cancer Res. 63 (19) :6110-6115 (2003) .
Background Reference 03
Saito, Y., et al., Int. J. Cancer 105 (4) :527-532 (2003) .
Background Reference 04
Siedlecki, P., et al., Biochem. Biophys. Res. Commun. 306 (2) :558-563 (2003) .
Other Product Names
DNA (cytosine-5) -methyltransferase 1, Dnmt1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, DNA MTase HsaI, MHsaI, MCMT, DNMT1, AIM, CXXC9, DNMT
Tested Applications
WB
Protein ID
12231019
Physical Properties
Liquid

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