DNMT1 Antibody

• Background:

  Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2Fbinding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells.

• NCBI Gene ID:

  1786

• Swiss Prot:

  P26358

• Accession Number:

  P26358

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This DNMT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1083-1112 amino acids from human DNMT1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  183 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  DNMT1

• NCBI Official Name:

  DNA (cytosine-5) -methyltransferase 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Peterson, E.J., et al., Cancer Res. 63 (20) :6579-6582 (2003) .

• Background Reference 02:

  Leu, Y.W., et al., Cancer Res. 63 (19) :6110-6115 (2003) .

• Background Reference 03:

  Saito, Y., et al., Int. J. Cancer 105 (4) :527-532 (2003) .

• Background Reference 04:

  Siedlecki, P., et al., Biochem. Biophys. Res. Commun. 306 (2) :558-563 (2003) .

• Other Product Names:

  DNA (cytosine-5) -methyltransferase 1, Dnmt1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, DNA MTase HsaI, MHsaI, MCMT, DNMT1, AIM, CXXC9, DNMT

• Tested Applications:

  WB

• Protein ID:

  12231019

• Physical Properties:

  Liquid