MAPK14 Antibody

• Background:

  MAPK14 encodes a protein which is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response.

• NCBI Gene ID:

  1432

• Swiss Prot:

  Q16539

• Accession Number:

  NP_001306

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MAPK14.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1309 - Human Placenta Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store MAPK14 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  41 kDa, 41 kDa

• Applications Notes:

  MAPK14 antibody can be used for detection of MAPK14 by ELISA at 1:62500. MAPK14 antibody can be used for detection of MAPK14 by western blot at 0.06 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  MAPK14

• NCBI Official Name:

  Mitogen-activated protein kinase 14

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Aleman, M., et al., (2004) Infect. Immun. 72 (9), 5150-5158.

• Other Product Names:

  MAPK14, RK, p38, CSBP, EXIP, Mxi2, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, SAPK2A, p38ALPHA

• Tested Applications:

  ELISA, WB, IHC

• Protein ID:

  4503069

• Physical Properties:

  Liquid