CDC25B Antibody

• Background:

  CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined.

• NCBI Gene ID:

  994

• Swiss Prot:

  P30305

• Accession Number:

  NP_068659

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CDC25B.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle, Cancer

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1224 - Daudi Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store CDC25B antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  65 kDa

• Applications Notes:

  CDC25B antibody can be used for detection of CDC25B by ELISA at 1:62500. CDC25B antibody can be used for detection of CDC25B by western blot at 1.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  CDC25B

• NCBI Official Name:

  Cell division cycle 25B

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Uchida, S., et al., (2004) Biochem. Biophys. Res. Commun. 316 (1), 226-232.

• Other Product Names:

  CDC25B, CDC25HU2

• Tested Applications:

  ELISA, WB, IHC

• Protein ID:

  11641413

• Physical Properties:

  Liquid