MDFIC Antibody

CAT: 0223-30-672Size: 100 µLDry Ice: NoHazardous: No
CAT#:0223-30-672Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
MDFIC is a member of a family of proteins characterized by a specific cysteine-rich C-terminal domain, which is involved in transcriptional regulation of viral genome expression. Alternative translation initiation from an upstream non-AUG (GUG), and an in-frame, downstream AUG codon, results in the production of two isoforms, p40 and p32, respectively, which have different subcellular localization; p32 is mainly found in the cytoplasm, whereas p40 is targeted to the nucleolus. Both isoforms have transcriptional regulatory activity that is attributable to the cysteine-rich C-terminal domain.This gene product is a member of a family of proteins characterized by a specific cysteine-rich C-terminal domain, which is involved in transcriptional regulation of viral genome expression. Alternative translation initiation from an upstream non-AUG (GUG), and an in-frame, downstream AUG codon, results in the production of two isoforms, p40 and p32, respectively, which have different subcellular localization; p32 is mainly found in the cytoplasm, whereas p40 is targeted to the nucleolus. Both isoforms have transcriptional regulatory activity that is attributable to the cysteine-rich C-terminal domain.
NCBI Gene ID
29969
Swiss Prot
Q9P1T7
Accession Number
NP_951038
Host
Rabbit
Reactivity
Human
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MDFIC.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Transcription
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
Cat. No. 1211 - HepG2 Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store MDFIC antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
37 kDa
Applications Notes
MDFIC antibody can be used for detection of MDFIC by ELISA at 1:1562500. MDFIC antibody can be used for detection of MDFIC by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
MDFIC
NCBI Official Name
MyoD family inhibitor domain containing
NCBI Organism
Homo sapiens
Background Reference 01
Gautier, V.W., (2005) Proc. Natl. Acad. Sci. U.S.A. 102 (45), 16362-16367.
Other Product Names
MDFIC, HIC
Tested Applications
ELISA, WB
Protein ID
40018612
Physical Properties
Liquid

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