FBXW7 Antibody

FBXW7 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in FBXW7 gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers.This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Three transcript variants encoding three different isoforms have been found for this gene.

55294

Q969H0-4

NP_001013433

Human, Mouse, Drosophila

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FBXW7.

Unconjugated

Primary Antibodies

Transcription, Cancer

Antibody is purified by peptide affinity chromatography method.

Cat. No. 1205 - Jurkat Cell Lysate

Batch dependent

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

None

Blue Ice

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store FBXW7 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

66 kDa

FBXW7 antibody can be used for detection of FBXW7 by ELISA at 1:312500. FBXW7 antibody can be used for detection of FBXW7 by western blot at 0.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

FBXW7

F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase

Homo sapiens

Lee, J.W., Pathology 40 (3), 305 (2008) .

FBXW7, AGO, CDC4, DKFZp686F23254, FBW7, FBX30, FBXW6, SEL-10, SEL10, FBW6, hAgo, hCdc4, FBXO30

ELISA, WB

61743926