FBXW5 Antibody

• Background :

  This gene encodes a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene contains WD-40 domains, in addition to an F-box motif, so it belongs to the Fbw class. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene, however, they were found to be nonsense-mediated mRNA decay (NMD) candidates, hence not represented.

• NCBI Gene ID :

  54461

• Swiss Prot :

  Q969U6

• Accession Number :

  Q969U6

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  This FBXW5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 324-350 amino acids from the Central region of human FBXW5.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Cell Cycle

• Purification :

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration :

  Batch dependent

• Buffer :

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight :

  64 kDa

• Fragment :

  Rabbit Ig

• Applications Notes :

  For WB starting dilution is: 1:1000

• Symbol :

  FBXW5

• NCBI Official Name :

  F-box/WD repeat-containing protein 5

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Davila, S., et al. Genes Immun. 11 (3) :232-238 (2010)

• Background Reference 02 :

  Minoda, Y., et al. Biochem. Biophys. Res. Commun. 381 (3) :412-417 (2009)

• Background Reference 03 :

  Hu, J., et al. Genes Dev. 22 (7) :866-871 (2008)

• Background Reference 04 :

  Wan, D., et al. Proc. Natl. Acad. Sci. U.S.A. 101 (44) :15724-15729 (2004)

• Other Product Names :

  F-box/WD repeat-containing protein 5, F-box and WD-40 domain-containing protein 5, FBXW5, FBW5

• Tested Applications :

  WB

• Protein ID :

  44887886

• Physical Properties :

  Liquid