ECHS1 Antibody

• Background:

  ECHS1 functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. ECHS1 is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix.The protein encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature.

• NCBI Gene ID:

  1892

• Swiss Prot:

  P30084

• Accession Number:

  NP_004083

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ECHS1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. XBL-10409 - Fetal Liver Tissue Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ECHS1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  28 kDa

• Applications Notes:

  ECHS1 antibody can be used for detection of ECHS1 by ELISA at 1:1562500. ECHS1 antibody can be used for detection of ECHS1 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  ECHS1

• NCBI Official Name:

  Enoyl CoA hydratase, short chain, 1, mitochondrial

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Bruneel, A., (2005) Proteomics 5 (15), 3876-3884.

• Other Product Names:

  ECHS1, SCEH

• Tested Applications:

  ELISA, WB

• Protein ID:

  194097323

• Physical Properties:

  Liquid