ECH1 Antibody

• Background:

  This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.

• NCBI Gene ID:

  1891

• Swiss Prot:

  Q13011

• Accession Number:

  Q13011

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This ECH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 223-250 amino acids from the C-terminal region of human ECH1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Obesity, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  36 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  ECH1

• NCBI Official Name:

  Delta (3,5) -Delta (2,4) -dienoyl-CoA isomerase, mitochondrial

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Olsen, J.V., et al. Cell 127 (3) :635-648 (2006)

• Background Reference 02:

  Olsen, J.V., et al. Cell 127 (3) :635-648 (2006)

• Background Reference 03:

  Kovalyov, L.I., et al. Biochemistry Mosc. 71 (4) :448-453 (2006)

• Background Reference 04:

  Goehler, H., et al. Mol. Cell 15 (6) :853-865 (2004)

• Other Product Names:

  Delta (3,5) -Delta (2,4) -dienoyl-CoA isomerase, mitochondrial, 533-, ECH1

• Tested Applications:

  WB

• Protein ID:

  82654933

• Physical Properties:

  Liquid