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UBE2J1 Antibody

CAT: 0223-29-845Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0223-29-845Size:100 µL
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Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2J1 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system.
NCBI Gene ID
51465
Swiss Prot
Q9Y385
Accession Number
NP_057105
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UBE2J1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Membrane
Purification
Antibody is purified by protein A chromatography method.
Positive Control
Cat. No. 1205 - Jurkat Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store UBE2J1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
35 kDa
Applications Notes
UBE2J1 antibody can be used for detection of UBE2J1 by ELISA at 1:1562500. UBE2J1 antibody can be used for detection of UBE2J1 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
UBE2J1
NCBI Official Name
Ubiquitin-conjugating enzyme E2, J1
NCBI Organism
Homo sapiens
Background Reference 01
Lenk, U., J. Cell. Sci. 115 (PT 14), 3007-3014 (2002) .
Other Product Names
UBE2J1, CGI-76, HSPC153, HSPC205, HSU93243, MGC12555, NCUBE1, Ubc6p, UBC6, NCUBE-1
Tested Applications
ELISA, WB, IHC
Protein ID
37577122
Physical Properties
Liquid

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