UBE2V1 Antibody

The CROC1 isoforms, also known as UBE2V1, show sequence similarity to ubiquitin-conjugating enzymes (UBCs, or E2s) but lack the conserved cysteine residue critical to catalytic activity of E2s.1 Northern blot analysis detected approximately 2.1- and 2.5-kb CROC1 transcripts in all human tissues examined, with the highest levels in brain, skeletal muscle, and kidney. Partial human intestinal epithelial cell cDNAs have been isolated containing the 3-primecoding sequence and 3-prime untranslated region of UBE2V1, also called UEV1.2 UEV1 does not have ubiquitin-conjugating activity in vitro. UEV1 transcripts are downregulated upon differentiation of a colon carcinoma cell line.1 Constitutive expression of exogenous UEV1 protein in these colon carcinoma cells inhibits their capacity to differentiate upon confluence and induces changes in cell cycle behavior associated with inhibition of CDK1. A heterodimeric protein complex has been identified that links TRAF6 to IKK activation.3 Peptide mass fingerprinting analysis revealed that this complex is composed of the ubiquitin conjugating enzyme UBC13 and the UBC-like protein UBE2V1, also called UEV1A. TRAF6, a RING domain protein, functions together with UBC13/UEV1A to catalyze the synthesis of unique polyubiquitin chains linked through lysine-63 (K63) of ubiquitin. Blockade of this polyubiquitin chain synthesis, but not inhibition of the proteasome, prevents the activation of IKK by TRAF6. These results unveil a new regulatory function for ubiquitin, in which IKK is activated through the assembly of K63-linked polyubiquitin chains.

3875227335

Q13404

Q13404

Human, Mouse

This UBE2V1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-145 amino acids from the C-terminal region of human UBE2V1.

Unconjugated

Primary Antibodies

Cell Cycle, Immunology, Signal Transduction

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Batch dependent

Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Zebrafish, Rat, Xenopus

Supplied in PBS with 0.09% (W/V) sodium azide.

None

Blue Ice

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

16 kDa

Rabbit Ig

For WB starting dilution is: 1:1000

UBE2V1

Ubiquitin-conjugating enzyme E2 variant 1

Homo sapiens

Thomson, T.M., et al., Genome Res. 10 (11) :1743-1756 (2000) .

Long, M., Genome Res. 10 (11) :1655-1657 (2000) .

Deng, L., et al., Cell 103 (2) :351-361 (2000) .

Hofmann, R.M., et al., Cell 96 (5) :645-653 (1999) .

Ubiquitin-conjugating enzyme E2 variant 1, UEV-1, CROC-1, TRAF6-regulated IKK activator 1 beta Uev1A, UBE2V1, CROC1, UBE2V, UEV1

WB

259016163