UBE2J2 Antibody

CAT: 0223-29-862Size: 100 µLDry Ice: NoHazardous: No
CAT#:0223-29-862Size:100 µL
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Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2J2 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.
NCBI Gene ID
118424
Swiss Prot
Q8N2K1
Accession Number
NP_919296
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UBE2J2.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Apoptosis, Homeostasis
Purification
Antibody is purified by protein A chromatography method.
Positive Control
Cat. No. 1211 - HepG2 Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store UBE2J2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
30 kDa, 29 kDa, 23 kDa, 23 kDa
Applications Notes
UBE2J2 antibody can be used for detection of UBE2J2 by ELISA at 1:312500. UBE2J2 antibody can be used for detection of UBE2J2 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
UBE2J2
NCBI Official Name
Ubiquitin-conjugating enzyme E2, J2
NCBI Organism
Homo sapiens
Background Reference 01
Otsuki, T., (2005) DNA Res. 12 (2), 117-126.
Other Product Names
UBE2J2, NCUBE2, PRO2121, NCUBE-2
Tested Applications
ELISA, WB, IHC
Protein ID
37577126
Physical Properties
Liquid

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