Actin alpha 2 Antibody

The alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. The two skeletal muscle isoforms of alpha-actinin (ACTN2 and ACTN3) are major structural components of the Z-line involved in anchoring the actin-containing thin filaments. In humans, ACTN2 is expressed in all muscle fibres, while ACTN3 expression is restricted to a subset of type 2 fibres. Murine Actn2 and Actn3 are differentially expressed, spatially and temporally, during embryonic development and, in contrast to humans, alpha-actinin-2 expression does not completely overlap alpha-actinin-3 in postnatal skeletal muscle, suggesting independent function.Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Transcript variants resulting from the use of multiple poly_A sites have been observed.

88

P35609

NP_001094

Human, Mouse, Rat, Dog

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human Actin alpha 2.

Unconjugated

Primary Antibodies

Other

Antibody is purified by protein A chromatography method.

Cat. No. XBL-10407 - Fetal Heart Tissue Lysate

Batch dependent

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

None

Blue Ice

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store Actin alpha 2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

98 kDa

Actin alpha 2 antibody can be used for detection of Actin alpha 2 by ELISA at 1:312500. Actin alpha 2 antibody can be used for detection of Actin alpha 2 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

ACTN2

Actinin, alpha 2

Homo sapiens

Eldstrom, J., FEBS Lett. 547 (1-3), 205-211 (2003) .

ACTN2, CMD1AA

ELISA, WB, IHC

4501893