TPM1 Antibody

TPM1 is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction.This gene is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The encoded protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy.

7168

P09493

NP_000357

Human, Mouse, Rat, Dog, Zebrafish

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TPM1.

Unconjugated

Primary Antibodies

Cancer

Antibody is purified by protein A chromatography method.

Cat. No. 1211 - HepG2 Cell Lysate

Batch dependent

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

None

Blue Ice

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store TPM1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

31 kDa, 33 kDa, 33 kDa, 33 kDa

TPM1 antibody can be used for detection of TPM1 by ELISA at 1:312500. TPM1 antibody can be used for detection of TPM1 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

TPM1

Tropomyosin 1 (alpha)

Homo sapiens

Lin, J.C. (2005) Mol. Cell. Biol. 25 (22), 10111-10121.

TPM1, CMH3, TMSA, TPM1-alpha, CMD1Y, LVNC9, C15orf13, HTM-alpha

ELISA, WB, IHC

27597085