TPM3 Antibody

• Background :

  TPM3 is a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Mutations in this gene result in autosomal dominant nemaline myopathy, and oncogenes formed by chromosomal translocations involving this locus are associated with cancer.This gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Mutations in this gene result in autosomal dominant nemaline myopathy, and oncogenes formed by chromosomal translocations involving this locus are associated with cancer. Multiple transcript variants encoding different isoforms have been found for this gene.

• NCBI Gene ID :

  7170

• Swiss Prot :

  P06753

• Accession Number :

  NP_705935

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TPM3.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Cancer

• Purification :

  Antibody is purified by peptide affinity chromatography method.

• Positive Control :

  Cat. No. 1208 - THP-1 Cell Lysate

• Concentration :

  Batch dependent

• Buffer :

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store TPM3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight :

  33 kDa

• Applications Notes :

  TPM3 antibody can be used for detection of TPM3 by ELISA at 1:312500. TPM3 antibody can be used for detection of TPM3 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol :

  TPM3

• NCBI Official Name :

  Tropomyosin 3

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Clarke, N.F., (2008) Ann. Neurol. 63 (3), 329-337.

• Other Product Names :

  TPM3, FLJ41118, MGC14582, MGC3261, MGC72094, NEM1, OK/SW-cl.5, TM-5, TM3, TM30, TM30nm, TPMsk3, TRK, hscp30, TM5, CFTD, CAPM1, HEL-189, HEL-S-82p

• Tested Applications :

  ELISA, WB

• Protein ID :

  24119203

• Physical Properties :

  Liquid