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SF1 Antibody

CAT: 0223-29-534Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0223-29-534Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
SF1 contains 1 CCHC-type zinc finger and 1 KH domain. SF1 is Necessary for the ATP-dependent first step of spliceosome assembly. It binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. SF1 may act as transcription repressor.This gene encodes a member of the heat shock factor (HSF) family of transcriptional activators for heat shock proteins. This gene is a candidate gene for azoospermia, since it localizes to a region of chromosome Y that is sometimes deleted in infertile males. The genome has two identical copies of this gene within a palindromic region; this record represents the more centromeric copy. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
NCBI Gene ID
7536
Swiss Prot
Q15637
Accession Number
NP_973724
Host
Rabbit
Reactivity
Human
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SF1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Transcription
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
Cat. No. 1205 - Jurkat Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SF1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
69 kDa
Applications Notes
SF1 antibody can be used for detection of SF1 by ELISA at 1:12500. SF1 antibody can be used for detection of SF1 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
SF1
NCBI Official Name
Splicing factor 1
NCBI Organism
Homo sapiens
Background Reference 01
Rino, J., (2008) Mol. Cell. Biol. 28 (9), 3045-3057.
Other Product Names
SF1, D11S636, ZFM1, ZNF162, BBP, MBBP, ZCCHC25
Tested Applications
ELISA, WB
Protein ID
42544125
Physical Properties
Liquid

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