DCP2 Antibody

• Background:

  DCP2 is necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. DCP2 removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. It has higher activity towards mRNAs that lack a poly (A) tail, but has no activity towards a cap structure lacking a RNA moiety.DCP2 is a key component of an mRNA-decapping complex required for removal of the 5-prime cap from mRNA prior to its degradation from the 5-prime end (Fenger-Gron et al., 2005 [PubMed 16364915]) .

• NCBI Gene ID:

  167227

• Swiss Prot:

  Q8IU60

• Accession Number:

  NP_689837

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DCP2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store DCP2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  48 kDa

• Applications Notes:

  DCP2 antibody can be used for detection of DCP2 by ELISA at 1:62500. DCP2 antibody can be used for detection of DCP2 by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  DCP2

• NCBI Official Name:

  Decapping mRNA 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Yamochi, T., (2008) Biochem. Biophys. Res. Commun. 370 (1), 195-199.

• Other Product Names:

  DCP2, FLJ33245, NUDT20

• Tested Applications:

  ELISA, WB

• Protein ID:

  31542498

• Physical Properties:

  Liquid