RNF8 Antibody

• Background:

  RNF8 contains a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins.The protein encoded by this gene contains a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

• NCBI Gene ID:

  9025

• Swiss Prot:

  O76064

• Accession Number:

  NP_003949

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RNF8.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store RNF8 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  55 kDa, 51 kDa

• Applications Notes:

  RNF8 antibody can be used for detection of RNF8 by ELISA at 1:312500. RNF8 antibody can be used for detection of RNF8 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  RNF8

• NCBI Official Name:

  Ring finger protein 8, E3 ubiquitin protein ligase

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Lehner, B. (2004) Genome Res. 14 (7), 1315-1323.

• Other Product Names:

  RNF8, FLJ12013, KIAA0646, hRNF8

• Tested Applications:

  ELISA, WB, IHC

• Protein ID:

  4504867

• Physical Properties:

  Liquid