CTCF Antibody

• Background :

  CTCF is a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT) -containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC) -containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in CTCF have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors.This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT) -containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC) -containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors.

• NCBI Gene ID :

  10664

• Swiss Prot :

  P49711

• Accession Number :

  NP_006556

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat, Dog

• Immunogen :

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CTCF.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Transcription, Cell Cycle, Cancer, Apoptosis

• Purification :

  Antibody is purified by peptide affinity chromatography method.

• Positive Control :

  Tranfected 293T Cell Lysate

• Concentration :

  Batch dependent

• Buffer :

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store CTCF antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight :

  83 kDa

• Applications Notes :

  CTCF antibody can be used for detection of CTCF by ELISA at 1:312500. CTCF antibody can be used for detection of CTCF by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol :

  CTCF

• NCBI Official Name :

  CCCTC-binding factor (zinc finger protein)

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Defossez, P.A., (2005) J. Biol. Chem. 280 (52), 43017-43023.

• Other Product Names :

  CTCF, MRD21

• Tested Applications :

  ELISA, WB

• Protein ID :

  5729790

• Physical Properties :

  Liquid