NFIL3 Antibody

• Background:

  NFIL3 is a basic leucine zipper (bZIP) transcription factor that represses or activates transcription in non-osteoblastic cells. NFIL3 play a role in attenuation of PTH target gene transcription in osteoblasts. NFIL3 repression domain interacts specifically with the TBP binding repressor protein Dr1.Glucocorticoid-mediated upregulation of the bZIP transcriptional repressor gene, NFIL3, is dependent on [Ca2+]i levels, and correlates with Glucocorticoid-evoked apoptosis of Glucocorticoid-sensitive CEM-C7-14 cells. NFIL3 is involved in a distinct growth factor-regulated signaling pathway that is responsible for the survival of early B-cell progenitors, and whose alteration by E2A-HLF leads to childhood B lineage leukemia.

• NCBI Gene ID:

  4783

• Swiss Prot:

  Q14211

• Accession Number:

  NP_005375

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFIL3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Tranfected 293T Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NFIL3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  51 kDa

• Applications Notes:

  NFIL3 antibody can be used for detection of NFIL3 by ELISA at 1:312500. NFIL3 antibody can be used for detection of NFIL3 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  NFIL3

• NCBI Official Name:

  Nuclear factor, interleukin 3 regulated

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ozkurt, I.C., et al., (2003) J. Biol. Chem. 278 (29), 26803-26809.

• Other Product Names:

  NFIL3, E4BP4, IL3BP1, NFIL3A, NF-IL3A

• Tested Applications:

  ELISA, WB

• Protein ID:

  52630429

• Physical Properties:

  Liquid