RUNX2 Antibody

• Background:

  The RUNX2 gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis, acting as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD) .

• NCBI Gene ID:

  860

• Swiss Prot:

  Q5T802

• Accession Number:

  Q5T802

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RUNX2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store RUNX2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  64 kDa, 54 kDa, 57 kDa, 55 kDa

• Applications Notes:

  RUNX2 antibody can be used for detection of RUNX2 by ELISA at 1:312500. RUNX2 antibody can be used for detection of RUNX2 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  RUNX2

• NCBI Official Name:

  Runt-related transcription factor 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ducy, P., et al., (1997) Cell. 89 (5), 747-54.

• Other Product Names:

  RUNX2, CCD, AML3, CCD1, CLCD, OSF2, CBFA1, OSF-2, PEA2aA, PEBP2aA, CBF-alpha-1

• Tested Applications:

  ELISA, WB

• Protein ID:

  74745461

• Physical Properties:

  Liquid