MCM2 Antibody

• Background:

  The protein encoded by MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.

• NCBI Gene ID:

  4171

• Swiss Prot:

  P49736

• Accession Number:

  NP_004517

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MCM2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle, Cancer

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store MCM2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  99 kDa

• Applications Notes:

  MCM2 antibody can be used for detection of MCM2 by ELISA at 1:312500. MCM2 antibody can be used for detection of MCM2 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  MCM2

• NCBI Official Name:

  Minichromosome maintenance complex component 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Yu, Z., et al., (2004) J. Mol. Biol. 340 (5), 1197-1206.

• Other Product Names:

  MCM2, BM28, CCNL1, CDCL1, cdc19, D3S3194, MITOTIN

• Tested Applications:

  ELISA, WB

• Protein ID:

  33356547

• Physical Properties:

  Liquid